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Product Name | Biotin ELISA |
Description | The Eagle Biosciences Biotin (Vitamin H) quantitative test is based on the principle of the enzyme linked immunosorbent assay. Avidin, which shows a high affinity to biotin, is bound on the surface of a microtiter plate. Biotin containing samples or standards and a biotin-alkaline phosphatase conjugate are given into the wells of the microtiter plate. Enzyme labelled and free biotin compete for the binding sites. After one hour incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A substrate solution is added and incubated for 30 minutes, resulting in the development of a yellow colour. The colour development is inhibited by the addition of a stop solution. The yellow colour is measured photometrically at 405 nm. The concentration of biotin is indirectly proportional to the colour intensity of the Eagle Biosciences Biotin ELISA Assay test sample. Sample types: food products. Range: 1.0 - 25 ng/ml. Sensitivity: 0.5 ng/mL. Incubation time: 1.5 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Biotin, Vitamin B7, Vitamin H, Coenzyme R test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | BIO06-K01 |
Price | $860 |
Order / More Info | Biotin ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |