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Product Name | SeroCP Quant C. pneumoniae IgG ELISA Assay Kit |
Description | The Eagle Biosciences SeroCP Quant Plates are supplied coated with purified elementary bodies of C.pneumoniae (TWAR - 183) antigens. The serum to be tested is diluted and incubated in the SeroCP Quant plate for 1h at 37°C. In this step C.pneumoniae antibodies are bound to the immobilized antigens. Non-specific antibodies are removed by washing. Anti-human IgG conjugated to Horseradish Peroxidase (HRP) is added and incubated 1h at 37°C. In this step the HRP-Conjugate is bound to the prebound antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB-Substrate, the Substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced Substrate. Upon the addition of the Stop Solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620nm. The absorbance is proportional to the amount of the specific antibodies that are bound to the coated antigens. Sample types: serum. Range: Cut-off. Sensitivity: Cut-off. Incubation time: 2.25 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | C. pneumoniae IgG, SeroCP, Quant test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | 291-01 |
Price | $495 |
Order / More Info | SeroCP Quant C. pneumoniae IgG ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |