Total FGF-21 ELISA Assay Kit from EAGLE BIOSCIENCES INC.
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| Product Name | Total FGF-21 ELISA Assay Kit |
| Description | This Eagle Biosciences Total FGF-21 ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human C-terminal FGF-21 in serum and EDTA-plasma sample. The assay utilizes the two-site ``sandwich'' technique with two selected antibodies that bind to different epitopes of human FGF-21. One of the antibodies specifically binds to the C-terminal human FGF-21 (175-181) and the other is to the multi-epitopes of mid-regional and N-terminal human FGF-21. Assay calibrators, controls and patient samples are added directly to wells of a microplate that is coated with an anti-human FGF-21 (175-181) specific antibody. After the first incubation period, a horseradish peroxidase-conjugated anti-human FGF-21 polyclonal antibody is added to each well. After the second incubation period, the antibody on the wall of microtiter well captures human N-terminal and C-terminal FGF-21 in the sample and further forms ``sandwich'' with the tracer antibody. Unbound proteins in each microtiter well are washed away. An immunocomplex of ``anti-FGF-21 antibody --- human N-terminal and C-terminal FGF-21 --- HRP-conjugated tracer antibody'' is formed. The unbound tracer antibody is removed in the subsequent washing step. For the detection of this immunocomplex, the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to human N-terminal and C-terminal FGF-21 on the wall of the microtiter well is directly proportional to the amount of N-terminal and C-terminal FGF-21 in the sample. A calibrator curve is generated by plotting the absorbance versus the respective human intact FGF-21 concentration for each calibrator on point-to-point or 4 parameter curve fit. The concentration of the combined N-terminal and C-terminal FGF-21 in test samples is determined directly from this calirbator curve. Sample types: serum, EDTA plasma. Range: 54 - 2100 pg/mL. Sensitivity: 10.4 pg/mL. Incubation time: 2.5 hours |
| Size | 1 x 96 well |
| Concentration | n/a |
| Applications | RUO |
| Other Names | Total Fibroblast Growth Factor 21, FGF-21, cnFGF-251 test, kit, assay, EIA, enzyme immunoassay |
| Gene, Accession, CAS # | n/a |
| Catalog # | T2131-K01 |
| Price | $715 |
| Order / More Info | Total FGF-21 ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110
info@EagleBio.com
http://www.EagleBio.com
Profile of EAGLE BIOSCIENCES INC.