Anti-Phospholipid screen ELISA from EAGLE BIOSCIENCES INC.
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| Product Name | Anti-Phospholipid screen ELISA |
| Description | The Eagle Biosciences Anti-Phospholipid Screen ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of IgG and / or IgM antibodies to negatively charged phospholipids. The antibodies of the standards, controls and diluted patient samples react with an Antigens complex consisting of cardiolipin, phosphatidyl-serine, -inositol, -ethanolamine, phosphatidic acid and the cofactor b2 GP-I immobilized on the solid phase of microtiter plates. The use of highly purified human b2 GP-I guarantees the specific binding of autoimmune related phospholipid antibodies of the specimen under investigation. Following an incubation period of 60 min at room temperature, unbound sample components are removed by a wash step.The bound antibodies react specifically with anti-human-IgG or anti-human-IgM conjugated to horseradish peroxidase (HRP) within the incubation period of 30 min at room temperature (RT). Excessive conjugate is separated from the solid-phase immune complexes by the following wash step.HRP converts the colorless substrate solution of 3,3',5,5'-tetramethyl benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature turning the solution from blue to yellow.The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the antibody concentrations of the calibrators (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve. Evaluating the test by a semi-quantitative method using a cut-off calibrator is also possible. Sample types: serum,. Range: 1-300 U/mL. Sensitivity: 1U/mL. Incubation time: 2.5 hours |
| Size | 1 x 96 well |
| Concentration | n/a |
| Applications | RUO |
| Other Names | PHS31-K01 ASSAY EIA ELISA IMMUNOASSAY KIT, Anti Phospholipid APAS test, kit, assay, EIA, enzyme immunoassay |
| Gene, Accession, CAS # | n/a |
| Catalog # | 4050 |
| Price | $435 |
| Order / More Info | Anti-Phospholipid screen ELISA from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110
info@EagleBio.com
http://www.EagleBio.com
Profile of EAGLE BIOSCIENCES INC.