Tyrosine Phosphatase-related Islet Antigen 2 AutoAb (IA2A) ELISA Assay Kit from EAGLE BIOSCIENCES INC.
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| Product Name | Tyrosine Phosphatase-related Islet Antigen 2 AutoAb (IA2A) ELISA Assay Kit |
| Description | In the IA2A ELISA, recombinant IA-2 protein coated onto plate wells can specifically recognize the IA-2 autoantibodies in human sera and calibrators. After a 16-20-hour incubation at 4?, IA-2 autoantibodies are captured by immobilized IA-2 protein while the excess or unbound components were discarded and washed away. Afterwards, biotinylated IA-2 protein (IA2-Biotin) is added for another round of incubation for 1 hour at 4? , wherein the IA2-Biotin detects IA-2 autoantibodies previously bound to IA-2 protein on the plate. After removal of nonspecific bindings, bound IA2-Biotin is revealed by addition of streptavidin horseradish peroxidase (STV-HRP), which specifically binds with biotin, followed by the substrate 3,3?,5,5?-Tetramethylbenzidine (TMB), which results in formation of a blue color. Color reaction will be further stopped by 2M H2SO4, transforming the blue color to yellow signals. The absorbance of yellow reaction mixture is measured by plate reader at 450nm and 405nm. The higher the reading is, the higher concentration of IA-2 autoantibodies. Low concentration of IA-2 autoantibodies (<15 u/ml) is recommended to be read off the 450nm calibration curve, while high value of IA-2 autoantibodies to be read off 405nm standard curve. The measuring interval is 5-2000U/ml (units are NIBSC 97/550). Sample types: serum. Range: 7.5 - 4000 U/mL. Incubation time: 16 - 21 hours 35 minutes |
| Size | 1 x 96 wells |
| Concentration | n/a |
| Applications | RUO |
| Other Names | Tyrosine Phosphatase-related Islet Antigen 2, IA2A test, kit, assay, EIA, enzyme immunoassay |
| Gene, Accession, CAS # | n/a |
| Catalog # | TPI31-K01 |
| Price | $725 |
| Order / More Info | Tyrosine Phosphatase-related Islet Antigen 2 AutoAb (IA2A) ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110
info@EagleBio.com
http://www.EagleBio.com
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