Edit |   |
---|---|
Product Name | Bovine (Cow) IL-6 ELISA |
Description | The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IL-6 present in samples reacts with the anti-IL-6 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection antibody, biotin conjugated anti-IL-6 is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IL-6 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IL-6 in the test sample. The quantity of IL-6 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Sample types: serum, plasma. Range: 15.63 - 1000 pg/mL. Sensitivity: 30 pg/ml. Incubation time: 130 minutes |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | IL-6 test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | BL661-K01 |
Price | $725 |
Order / More Info | Bovine (Cow) IL-6 ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |