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Product Name | Human KIM-1 ELISA |
Description | The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the KIM-1 present in samples reacts with the anti- KIM-1 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-KIM-1, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of KIM-1 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of KIM-1 in the test sample. The quantity of KIM-1 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Sample types: serum, plasma, urine. Range: 0.125 ng/ml - 4 ng/ml. Sensitivity: 0.054 ng/ml. Incubation time: 110 minutes |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | KIM-1 test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | HKM39-K01 |
Price | $725 |
Order / More Info | Human KIM-1 ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |