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Product Name | SeroCP C. pneumoniae IgA ELISA Assay Kit |
Description | The Eagle Biosciences SeroCP Plates are supplied coated with purified elementary bodies of C. pneumoniae (TWAR 183) antigens. * The serum to be tested is diluted and incubated in the SeroCP plate for 1h at 37°C. In this step C.pneumoniae antibodies are bound to the immobilized antigens. * Non-specific antibodies are removed by washing. * Anti-human IgA conjugated to Horseradish Peroxidase (HRP) is added and incubated 1h at 37°C. In this step the HRP-Conjugate is bound to the prebound antigen-antibody complex. * Unbound conjugate is removed by washing. * Upon the addition of TMB-Substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced Substrate. * Upon the addition of the Stop Solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450nm. * The absorbance is proportional to the amount of the specific antibodies that are bound to the coated antigens. Sample types: serum. Range: Cut-off. Sensitivity: Cut-off. Incubation time: 2.25 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | C. pneumoniae IgA, SeroCP test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | 193-01 |
Price | $450 |
Order / More Info | SeroCP C. pneumoniae IgA ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |