Nitrotyrosin (EDTA-Plasma) ELISA Assay Kit from EAGLE BIOSCIENCES INC.
Search, find, compare suppliers for Nitrotyrosin (EDTA-Plasma) ELISA Assay Kit products.
| Edit | |
|---|---|
| Product Name | Nitrotyrosin (EDTA-Plasma) ELISA Assay Kit |
| Description | This ELISA is designed for the quantitative determination of nitrotyrosine. The assay utilises the ``sandwich'' technique. Standards, controls and prepared samples which are assayed for nitrotyrosine are added into the wells of a micro plate coated with polyclonal goat anti- nitrotyrosine antibody. During the first incubation step, nitrated proteins are bound by the immo-bilised primary antibody. Then a peroxidase-conjugated polyclonal goat anti-human serum proteins antibody is added into each microtiter well and a ``sandwich'' of primary antibody - nitrated protein - peroxidase-conjugate is formed. Tetramethylbenzidine is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The colour changes from blue to yellow. The intensity of the yellow colour is directly proportional to the concentration of nitrotyrosine. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. standard concentration is generated, using the values obtained from the standards. Sample types: EDTA plasma, serum, dried blood spots. Range: 4.9-400 nM. Incubation time: 2.25 hours |
| Size | 1 x 96 well |
| Concentration | n/a |
| Applications | RUO |
| Other Names | kit, assay, EIA, enzyme immunoassay |
| Gene, Accession, CAS # | n/a |
| Catalog # | KR7829 |
| Price | $1190 |
| Order / More Info | Nitrotyrosin (EDTA-Plasma) ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110
info@EagleBio.com
http://www.EagleBio.com
Profile of EAGLE BIOSCIENCES INC.