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Product Name | SeroCP RT C. pneumoniae IgA ELISA Assay Kit |
Description | The Eagle Biosciences SeroCP RT Plates are coated with C. pneumoniae specific antigens. Serum to be tested is diluted and incubated with the pre-coated SeroCPTM RT plate 30 minutes at Room Temperature (RT). In this step C. pneumoniae specific antibodies are bound to the immobilized C. pneumoniae specific antigens. Non-specific antibodies are removed by washing. Anti-human IgA conjugated to horseradish peroxidase (HRP) is added and incubated 30 minutes at Room Temperature. In this step the HRP-conjugate is bound to the pre-formed antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620 nm. The absorbance is proportional to the amount of the specific antibodies which are bound to the immobilized C. pneumoniae specific antigens. Sample types: serum. Range: Cut-off. Sensitivity: Cut-off. Incubation time: 1.5 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | C. pneumoniae IgA, SeroCP RT test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | 1193-01 |
Price | $495 |
Order / More Info | SeroCP RT C. pneumoniae IgA ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |