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Product Name | Human Serpin A7 (TBG) ELISA Kit |
Description | The Eagle Biosciences Human SERPIN A7 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human SERPINA7 with a 96-well strip plate that is pre-coated with antibody specific for SERPINA7. The detection antibody is a biotinylated antibody specific for SERPINA7. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human SERPINA7 with immunogen: Expression system for standard: NSO; Immunogen sequence: A21-A415. The kit is analytically validated with ready to use reagents. To measure Human SERPINA7, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human SERPINA7 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human SERPINA7 in the sample. Sample types: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Range: 156 pg/mL - 10000 pg/mL. Sensitivity: <10pg/ml. Incubation time: 2 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Serpin test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | TBG31-K01 |
Price | $585 |
Order / More Info | Human Serpin A7 (TBG) ELISA Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |