Human HMGB1 ELISA Kit from EAGLE BIOSCIENCES INC.

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Product NameHuman HMGB1 ELISA Kit
DescriptionThis assay employs the sandwich enzyme immunoassay technique for the detection of Human HMGB1 in plasma and cell culture supernatants samples. An antibody specific for HMGB1 has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and then a Horseradish Peroxidase (HRP) conjugated primary antibody binds to HMGB1 is added to each well and incubates. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of total HMGB1 bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm. The concentration of total HMGB1 in the sample is then determined by comparing the O.D of samples to the standard curve. Sample types: plasma, cell culture supernatants. Range: 0.3125-20 ng/ml. Sensitivity: 0.3 ng/ml. Incubation time: overnight
Size1 x 96 well
Concentrationn/a
ApplicationsRUO
Other NamesHMGB1 test, kit, assay, EIA, enzyme immunoassay
Gene, Accession, CAS #n/a
Catalog #ARG81185
Price$1640
Order / More InfoHuman HMGB1 ELISA Kit from EAGLE BIOSCIENCES INC.
Product Specific Referencesn/a
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110

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