Glutamic Acid Decarboxylase AutoAb (GADA) ELISA Kit from EAGLE BIOSCIENCES INC.
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| Product Name | Glutamic Acid Decarboxylase AutoAb (GADA) ELISA Kit |
| Description | In this GADA ELISA, recombinant GAD protein coated onto plate wells can specifically recognize the GAD autoantibodies in human sera and calibrators. After a 1-hour incubation, GAD autoantibodies are captured by immobilized GAD protein while the unbound components were discarded and washed away. Afterwards, biotinylated GAD protein (GAD Biotin) is added for another round of incubation for 1 hour, wherein the GAD-Biotin detects GAD autoantibodies previously bound to GAD protein on the plate. After removal of nonspecific bindings, bound GAD-Biotin is revealed by addition of streptavidin horseradish peroxidase (STV-HRP), which specifically binds with biotin, followed by the substrate 3,3?,5,5?-Tetramethylbenzidine (TMB), which results in formation of a blue color. Color reaction will be further stopped by 2M H2SO4, transforming the blue color to yellow signals. The absorbance of yellow reaction mixture is measured by plate reader at 450nm and 405nm. The higher the reading is, the higher concentration of GAD autoantibodies. Low concentration of GAD autoantibodies (<18 u/ml) is recommended to be read off the 450nm calibration curve, while high value of GAD Glutamic Acid Decarboxylase (GAD) ELISA Assay Kit 2/10 Catalog Number: GDA31-K01 www.EagleBio.com autoantibodies to be read off 405nm standard curve. The measuring interval is 5-2000U/ml (units are NIBSC 97/550). Sample types: serum. Range: 5 - 2000 U/ml. Incubation time: 2 hours 35 minutes |
| Size | 1 x 96 wells |
| Concentration | n/a |
| Applications | RUO |
| Other Names | Glutamic Acid Decarboxylase, GAD test, kit, assay, EIA, enzyme immunoassay |
| Gene, Accession, CAS # | n/a |
| Catalog # | GDA31-K01 |
| Price | $725 |
| Order / More Info | Glutamic Acid Decarboxylase AutoAb (GADA) ELISA Kit from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110
info@EagleBio.com
http://www.EagleBio.com
Profile of EAGLE BIOSCIENCES INC.