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Product Name | Human PAI-1 ELISA Assay Kit |
Description | This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a mouse monoclonal antibody specific for human PAI-1. Standards and samples are pipetted into the wells and any human PAI-1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human PAI-1 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human PAI-1 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human PAI-1, the unknown sample concentration can be interpolated from a reference curve included in each assay. Sample types: serum/plasma. Range: 0.031-2 ng/ml. Sensitivity: 0.031 ng/ml. Incubation time: 2 hrs 35 mins |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Plasminogen Activator Inhibitor-1, PAI-1 test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | PAI31-K01 |
Price | $615 |
Order / More Info | Human PAI-1 ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |