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Product Name | Free Estriol Saliva ELISA Assay Kit |
Description | The Free Estriol (also referred to as unconjugated estriol or uE3 in the literature) ELISA is a competitive immunoassay. Competition occurs between Estriol present in calibrators, controls, and samples and an enzyme-labeled antigen (conjugate) for a limiting number of anti-Estriol antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the enzyme substrate is added, and approximately 15-20 minutes late the enzymatic reaction is terminated by addition of stopping solution.The resulting optical density (OD), measured with a microplate reader, is inversely proportional to the concentration of Estriol in the sample. A calibrator curve is plotted with a provided set of calibrators to calculate directly the concentration of estriol in samples and controls. Serum and salivary assays follow the same procedure except that the volume of the calibrators, controls, and sample dispensed into the microplate wells in 10µL for serum assays and 20µLfor salivary assays. Sample types: saliva, serum. Range: 0.05 - 30 ng/mL. Sensitivity: 0.0058 ng/mL. Incubation time: 1 hour 20 minutes |
Size | 12 x 8 wells |
Concentration | n/a |
Applications | RUO |
Other Names | free estriol test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | FES32-K01 |
Price | $265 |
Order / More Info | Free Estriol Saliva ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |