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Product Name | Rat GST alpha ELISA |
Description | The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the AGST present in samples reacts with the anti-AGST antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-AGST antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound AGST. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of AGST in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of AGST in the test sample. The quantity of AGST in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Sample types: serum, plasma. Range: 3.125 ng/ml - 100 ng/ml. Sensitivity: 1.473 ng/ml. Incubation time: 190 minutes |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | GST alpha test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | RGT21-K01 |
Price | $635 |
Order / More Info | Rat GST alpha ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |