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Product Name | IAA (Insulin Autoantibodies) ELISA |
Description | In the first step Insulin AAb from the diluted sample bind to human recombinant insulin coated on the microtiter plate. After an incubation of 60 minutes at 37 °C unbound components are removed by washing. In a next step bound antibodies reacts with the added anti-human-IgG horseradish peroxidase (HRP) complex. Excessive conjugate is removed after 15 minutes at 37 °C by another washing step. HRP converts the colorless substrate TMB added into a blue product. The enzyme reaction is stopped by adding an acid solution after 15 minutes at 37 °C. The absorbance of the resulting yellow product is measured at 450 / 620 nm within 30 minutes. The obtained OD is direct proportional to the amount of bound antibodies. Sample types: serum. Range: 0.1 - 20 U/mL. Sensitivity: 0.08 U/mL. Incubation time: 90 minutes |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | IAA, Insulin Autoantibody, antibodies test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | 3806 |
Price | $595 |
Order / More Info | IAA (Insulin Autoantibodies) ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |