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Product Name | Rat IgG (high sensitive) ELISA Kit |
Description | This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for IgG has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any IgG present is bound by the immobilized antibody. After washing away any unbound substances, a HRP-conjugated antibody specific for IgG is added to each well and incubate. Following a washing to remove unbound substances, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of IgG bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm.The concentration of IgG in the sample is then determined by comparing the O.D of samples to the standard curve. Sample types: serum, plasma and cell culture supernatants. Range: 1.56 - 100 ng/ml. Sensitivity: 0.8 ng/ml. Incubation time: 3.5 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | IgG test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | ARG81184 |
Price | $715 |
Order / More Info | Rat IgG (high sensitive) ELISA Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |