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Product Name | Ficolin-3, Human, ELISA kit, 2 x 96 det. |
Description | The human Ficolin-3 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 31/2 hours. The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured Ficolin-3. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the Ficolin-3 standards (log). The human Ficolin-3 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve. Sample types: serum, plasma, cell culture supernatants. Range: 3-500 ng/ml. Sensitivity: 3 ng/ml. Incubation time: 3.5 hours |
Size | 2 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | FIC39-K02 |
Price | $1395 |
Order / More Info | Ficolin-3, Human, ELISA kit, 2 x 96 det. from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |