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Product Name | Vitamin B12 ELISA |
Description | The Eagle Biosciences Vitamin B12 quantitative test is based on the principle of the enzyme linked immunosorbent assay. An antibody directed against vitamin B12 is bound on the surface of a microtiter plate. Vitamin B12 containing samples or standards and a vitamin B12-peroxidase conjugate are given into the wells of the microtiter plate. Enzyme labelled and free vitamin B12 compete for the antibody binding sites. After one hour incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A substrate solution is added and incubated for 20 minutes, resulting in the development of a blue colour. The colour development is inhibited by the addition of a stop solution, and the colour turns yellow. The yellow colour is measured photometrically at 450 nm. The concentration of vitamin B12 is indirectly proportional to the colour intensity of the test sample. Sample types: food products. Range: 0.4 - 40 ng/ml. Sensitivity: 0.3 ng/ml. Incubation time: 1.5 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Cobalamin, Vitamin B12, B12 test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | B1206-K01 |
Price | $860 |
Order / More Info | Vitamin B12 ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |