Human Anti-histones ELISA (IgG) from EAGLE BIOSCIENCES INC.
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| Product Name | Human Anti-histones ELISA (IgG) |
| Description | The determination of anti-histone antibodies is based on an indirect enzyme linked immune reaction. The microtiter plate is pre-coated with purified total histones, which bind to the anti-histone antibodies present in the standards and samples. After incubation and washing, any unbound antibodies will be removed. Then goat anti-human IgG horseradish peroxidase (HRP) conjugates are added, which bind to the captured anti histone antibodies. After incubation and washing, any unbound conjugates will be also removed. Then substrate is catalyzed by the HRP to produce a blue color that changes to yellow after adding the stopping buffer. The density of the yellow coloration is directly proportional to the amount of captured anti-histone antibodies in the plate. The light absorbance (OD value) under 450nm wavelength of the wells is determined using a microplate reader. The antibody concentration of the unknown sample can be estimated with the provided calibrators in the kit. Since no international standard has been established for anti-histone antibodies, the standards are calibrated against Anti-Nuclear Factor Serum (Homogeneous) Human (NIBSC code: W1064, Human Anti-Histones (IgG) ELISA Assay Kit 2/9 Catalog Number: HST31-K01 www.EagleBio.com non-WHO reference material), and presented as relevant unit (RU) per mL. The kit offers semiquantitative and quantitative interpretation of the data, which is in the section of DATA INTERPRETATION. Sample types: serum, plasma. Incubation time: 1 hour 40 minutes |
| Size | 1 x 96 wells |
| Concentration | n/a |
| Applications | RUO |
| Other Names | Anti-Histones IgG test, kit, assay, EIA, enzyme immunoassay |
| Gene, Accession, CAS # | n/a |
| Catalog # | HTS31-K01 |
| Price | $545 |
| Order / More Info | Human Anti-histones ELISA (IgG) from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110
info@EagleBio.com
http://www.EagleBio.com
Profile of EAGLE BIOSCIENCES INC.