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Product Name | Pichia Pastoris HCP ELISA |
Description | This assay is based on the Sandwich ELISA procedure. Samples containing P.pastoris HCPs are reacted with already coated affinity purified capture anti-P.pastoris antibody and bind to them. Plates are washed with wash buffer to remove unbound reactants. Anti P.pastoris-HRP conjugate is added which results in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The wells are washed to remove any unbound reactants as per the wash procedure. The substrate 3, 3',5, 5' Tetra Methyl Benzidine is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and it is directly proportional to the concentration of P. pastoris HCPs present. Sample types: cell culture supernatant and other biological preparations. Range: 15.6 - 1000 ng/mL. Sensitivity: 15 ng/mL. Incubation time: 3 hours 45 minutes |
Size | 1 x 96 wells |
Concentration | n/a |
Applications | RUO |
Other Names | Pinchia Pastoris HCP test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | KBBP02 |
Price | $610 |
Order / More Info | Pichia Pastoris HCP ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |