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Product Name | Swine IL-4 ELISA KIT |
Description | The Eagle Biosciences Swine Interleukin 2 (IL-2) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-4 has been pre-coated onto a microplate. Standard, control, or sample and the working solution of Biotin-Conjugate are pipetted into the wells. Following incubation and wash steps any IL-4 present is bound by the immobilized antibody and the detection antibody specific for IL-4 is binds to the combination of capture antibody-IL-4 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-4 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-4 standard dilutions and IL-4 sample concentration determined. Sample types: serum, plasma, cell culture. Range: 15.625 - 500 pg/ml. Sensitivity: 7 pg/ml. Incubation time: 3.5 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Interleukin 4, IL-4 test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | IL451-K01 |
Price | $505 |
Order / More Info | Swine IL-4 ELISA KIT from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |