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Product Name | Human Serum Amyloid A (SAA) ELISA |
Description | The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed f rom the standards, and corrected for sample dilution. Sample types: serum, plasma. Range: 1.56 ng/ml - 100 ng/ml. Sensitivity: 0.492 ng/ml. Incubation time: 90 minutes |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Serum Amyloid A (SAA) ELISA test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | HSA31-K01 |
Price | $600 |
Order / More Info | Human Serum Amyloid A (SAA) ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |