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Product Name | Urinary Isoprostane EIA Kit |
Description | This Urinary Isoprotane ELISA kit is a competitive enzyme-linked immunoassay (ELISA) for determining levels of 15-isoprostane F2t (the best characterized isoprostane) in urine samples. Briefly, urine samples are mixed with an enhanced dilution buffer that essentially eliminates interference due to non-specific binding. The 15-isoprostane F2t in the samples or standards competes with 15-isoprostane F2t conjugated to horseradish peroxidase (HRP) for binding to a polyclonal antibody specific for 15-isoprostane F2t coated on the microplate. The HRP activity results in color development when substrate is added, with the intensity of the color proportional to the amount of 15-isoprostane F2t-HRP bound and inversely proportional to the amount of unconjugated 15-isoprostane F2t in the samples or standards. Sample types: urine. Range: 0.05 - 100 ng/mL. Sensitivity: 0.05 ng/mL. Incubation time: 2.5 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | 15-Isoprostane F2t, 8-epi-PGF2 Alpha, 8-iso-PGF2 Alpha, 8-isoprostane test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | ISO34-K01 |
Price | $545 |
Order / More Info | Urinary Isoprostane EIA Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |