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Product Name | Testosterone - Free CLIA |
Description | The principle of the following chemiluminescence immunoassay (LIA) test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, control and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microplate. The washing and decanting procedures remove unbound materials. After the washing step, the luminescence substrate solution is added. The relative luminescence units (RLUs) are measured on a microtiter plate luminometer. The RLU values are inversely proportional to the concentration of free testosterone in the sample. A set of calibrators are used to plot a standard curve from which the amount of free testosterone in patient samples and controls can be directly read. The labelled testosterone (conjugate) employed in this assay system has shown no binding properties towards SHBG and human serum albumin. A highly specific rabbit anti-testosterone polyclonal antibody at a low binding capacity (Keq x concentration) is used to keep minimum disturbances of the testosterone-protein equilibrium. The other components in the test system are also optimized in order to not alter the original free testosterone concentration. Sample types: serum. Range: 0.25-125 pg/mL. Sensitivity: 0.17 pg/mL. Incubation time: 90 minutes |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Testosterone test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | TSF31-L01 |
Price | $215 |
Order / More Info | Testosterone - Free CLIA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |