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Product Name | Human Apolipoprotein CII ELISA Kit |
Description | This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for ApoCII has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any ApoCII present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated antibody specific for ApoCII is added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibodyenzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of ApoCII bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm. The concentration of ApoCII in the sample is then determined by comparing the O.D of samples to the standard curve. Sample types: plasma, serum or other biological fluids. Range: 1.56 - 100 ng/ml. Sensitivity: 1 ng/ml. Incubation time: 4.5 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Apolipoprotein CII, APO CII test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | ARG81209 |
Price | $1360 |
Order / More Info | Human Apolipoprotein CII ELISA Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |