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Product Name | Chorionic Gonadotropin (hCG) ELISA |
Description | The principle of the following enzyme immunoassay test follows a typical one-step capture or `sandwich' type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for hCG is immobilized onto the microplate and another monoclonal antibody specific for a different region of hCG is conjugated to horse radish peroxidase (HRP). hCG from the sample and standards are allowed to bind simultaneously to the plate and to the HRP conjugate. The washing and decanting steps remove any unbound HRP conjugate. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of hCG in the sample. A set of standards is used to plot a standard curve from which the amount of hCG in patient samples and controls can be directly read. Please note that a two-step procedure is included and is to be used for assaying serum from pregnant women. Sample types: serum. Range: 2.5-500 IU/L. Sensitivity: 0.7 IU/L. Incubation time: 1.25 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | hCG test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | HCG31-K01 |
Price | $215 |
Order / More Info | Chorionic Gonadotropin (hCG) ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |