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Product Name | FGF-19 ELISA |
Description | This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a rabbit polyclonal antibody specific for human FGF-19. Standards and samples are pipetted into the wells and any human FGF-19 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human FGF-19 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human FGF-19 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human FGF-19, the unknown sample concentration can be interpolated from a reference curve included in each assay. Sample types: serum/plasma/cell culture supernates. Range: 31.2 - 2000 pg/ml. Sensitivity: 31.2 pg/ml. Incubation time: 2 hrs 35 mins |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | FGF-19, Fibroblast growth factor 19 test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | FBR31-K01 |
Price | $725 |
Order / More Info | FGF-19 ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |