Human BDNF ELISA Kit from EAGLE BIOSCIENCES INC.

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Product NameHuman BDNF ELISA Kit
DescriptionThe Eagle Biosciences BDNF (Brain-Derived Neurotrophic Factor) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for BDNF has been pre-coated onto a microtiter plate. Standards or samples are pipetted into the wells and any BDNF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated antibody specific for BDNF is added to each well and incubate. Following a washing to remove unbound substances, streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After washing away any unbound antibody-enzyme reagent, a substrate solution (TMB) is added to the wells and color develops in proportion to the amount of BDNF bound in the initial step. The color development is stopped by the addition of acid and the intensity of the color is measured at a wavelength of 450nm ±2nm.The concentration of BDNF in the sample is then determined by comparing the O.D of samples to the standard curve. Sample types: serum, plasma, cell culture supernatants. Range: 15.625-1000 pg/ml. Sensitivity: 7 pg/mL. Incubation time: 3.5 hours
Size1 x 96 well
Concentrationn/a
ApplicationsRUO
Other NamesBDNF, Brain-Derived Neurotrophic Factor test, kit, assay, EIA, enzyme immunoassay
Gene, Accession, CAS #n/a
Catalog #ARG81251
Price$715
Order / More InfoHuman BDNF ELISA Kit from EAGLE BIOSCIENCES INC.
Product Specific Referencesn/a
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110

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