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Product Name | TIMP-1 ELISA Assay Kit |
Description | This assay is a quantitative sandwich ELISA. The immunoplate is pre-coated with a monoclonal antibody specific for human TIMP-1. Standards and samples are pipetted into the wells and any human TIMP-1 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for human TIMP-1 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and colour develops in proportion to the amount of human TSP-2 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured human TIMP-1, the unknown sample concentration can be interpolated from a reference curve included in each assay. Sample types: serum/plasma/cell culture supernates. Range: 31.2pg/ml - 2000pg/ml. Sensitivity: 31.2pg/ml. Incubation time: 3 hrs 35 mins |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Tissue Inhibitor of Metalloproteinases-1, TIMP-1 test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | TMP31-K01 |
Price | $635 |
Order / More Info | TIMP-1 ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |