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Product Name | MPO, Rat, ELISA kit, 1 x 96 det. |
Description | The rat MPO ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 31/2 hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are captured by a solid bound specific antibody. Biotinylated tracer antibody will bind to captured rat MPO. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of citric acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the rat MPO standards (log). The rat MPO concentration of samples, which are run concurrently with the standards, can be determined from the standard curve. Sample types: plasma, tissue homogenate and cell culture supernatant. Range: 3.9-250 ng/ml. Sensitivity: 3.9 ng/ml. Incubation time: 3.5 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | RMP29-K01 |
Price | $1025 |
Order / More Info | MPO, Rat, ELISA kit, 1 x 96 det. from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |