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Product Name | SeroCT RT C. trachomatis IgG ELISA Assay Kit |
Description | SeroCT RT Plates are coated with C. trachomatis specific peptides. Serum to be tested is diluted and incubated with the pre-coated SeroCT RT plate 30 minutes at Room Temperature (RT). In this step C. trachomatis specific antibodies are bound to the immobilized C. trachomatis peptides. Non-specific antibodies are removed by washing. Anti-human IgG conjugated to horseradish peroxidase (HRP) is added and incubated 30 minutes at Room Temperature. In this step the HRP-conjugate is bound to the prebound antigen-antibody complex. Unbound conjugate is removed by washing. Upon the addition of TMB substrate, the substrate is hydrolyzed by the peroxidase, yielding a blue solution of the reduced substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620 nm. The absorbance is proportional to the amount of the specific antibodies which are bound to the immobilized peptides. Sample types: serum. Range: Cut-off. Sensitivity: Cut-off. Incubation time: 1.5 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Chlamydia IgG, SeroCT RT test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | 1181-01 |
Price | $495 |
Order / More Info | SeroCT RT C. trachomatis IgG ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |