Anti-β2 GP-I (Beta 2 Glycoprotein 1) ELISA from EAGLE BIOSCIENCES INC.

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Product NameAnti-β2 GP-I (Beta 2 Glycoprotein 1) ELISA
DescriptionThe Eagle Biosciences Anti- β2 GP-I ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of IgG or IgM antibodies to β2 glycoprotein-I in human serum or plasma. The antibodies of the standards, the positive control and diluted patient samples react with the human β2 GP-I, immobilized on the solid phase of microtiter plates. The use of highly purified β2 GP-I guarantees the specific binding of antibodies to β2 glycoprotein-I of the specimen under investigation. Following an incubation period of 60 min at room temperature (RT), unbound serum components are removed by a wash step.The bound antibodies react specifically with anti-human-IgG or IgM conjugated to horseradish peroxidase (HRP) within the next incubation period of 30 min at RT. Excessive conjugate is separated from the solid-phase immune complexes by an additional wash step. HRP converts the colorless substrate solution of 3,3?,5,5?-tetramethyl-benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an added acidic solution (H2SO4) into the wells, after 15 min at RT turning the solution from blue to yellow.The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specifically bound antibodies.The standard curve is established by plotting the antibody concentrations of the standards (x-axis) and their corresponding OD-values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve. Sample types: serum, plasma. Range: 1-300 U/mL. Sensitivity: 1 U/ml. Incubation time: 2.5 hours
Size1 x 96 well
Concentrationn/a
ApplicationsRUO
Other NamesB2G31-K01 ASSAY EIA ELISA IMMUNOASSAY KIT Anti Beta Glycoprotein 1 GP1,APAS, anti phospholipid test, kit, assay, EIA, enzyme immunoassay
Gene, Accession, CAS #n/a
Catalog #4041
Price$435
Order / More InfoAnti-β2 GP-I (Beta 2 Glycoprotein 1) ELISA from EAGLE BIOSCIENCES INC.
Product Specific Referencesn/a
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110

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