Human Anti-IgE ELISA Assay Kit from EAGLE BIOSCIENCES INC.
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| Product Name | Human Anti-IgE ELISA Assay Kit |
| Description | This Eagle Biosciences Human Anti-IgE Antibody ELISA Assay Kit is designed, developed and produced for the quantitative measurement of human anti-hIgE autoantibodies in serum and plasma samples. The assay utilizes the two-site ``bridge'' technique with two selected human antibodies. Assay standards, controls and patient samples are directly added to wells of a microplate that is coated with purified human IgE. After the first incubation period, anti-IgE antibodies bind to the human IgE on the wall of microtiter well and unbound proteins in each microtiter well are washed away. A highly purified biotin-labeled human IgE is then added to each microtiter well. After the second incubation period, and a ``bridge'' of ``well coated human IgE = Anti-hIgE antibody = biotin-labeled human IgE'' is formed. The unbound protein is removed in the subsequent washing step. HRP-labeled streptavidin is added to the plate wells. For the detection of this immunocomplex, the wells are then incubated with a substrate solution in a timed reaction and subsequently measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to the IgE on the wall of the microtiter well is directly proportional to the amount of anti-IgE antibody in the sample. A standard curve is generated by plotting the absorbance versus the respective anti-IgE concentration for each standard on point-to-point or 4 parameter curve fit. The concentration of IgE antibody in test samples is determined directly from this standard curve. Sample types: serum, plasma. Range: 12 - 450 ng/mL. Sensitivity: 0.4808 ng/mL. Incubation time: 3 hours |
| Size | 1 x 96 well |
| Concentration | n/a |
| Applications | RUO |
| Other Names | IgE ELISA test, kit, assay, EIA, enzyme immunoassay |
| Gene, Accession, CAS # | n/a |
| Catalog # | HAE31-K01 |
| Price | $640 |
| Order / More Info | Human Anti-IgE ELISA Assay Kit from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110
info@EagleBio.com
http://www.EagleBio.com
Profile of EAGLE BIOSCIENCES INC.