RF IgM (Rheumatoid Factor) ELISA from EAGLE BIOSCIENCES INC.
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| Product Name | RF IgM (Rheumatoid Factor) ELISA |
| Description | RF IgM is an enzyme immunoassay for the quantitative determination of IgM antibodies to the Fc region of IgG in human serum or plasma. The rheumatoid factors of the standards, control and diluted patient samples react with rabbit IgG immobilized on the solid phase of microtiter plates. Following an incubation period of 60 min at room temperature, unbound sample components are removed by a wash step.The bound IgG antibodies react specifically with anti-human-IgA conjugated to horseradish peroxidase (HRP). Within the incubation period of 30 min at RT, excessive conjugate is separated from the solid-phase immune complexes by the following wash step.HRP converts the colorless substrate solution of 3,3',5,5'-tetramethylbenzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 min at room temperature turning the solution from blue to yellow.The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the antibody concentrations of the calibrators (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curves. Sample types: serum, plasma. Range: 1-300 U/mL. Sensitivity: 1U/mL. Incubation time: 2.5 hours |
| Size | 1 x 96 well |
| Concentration | n/a |
| Applications | RUO |
| Other Names | RFM31-K01 ASSAY EIA ELISA IMMUNOASSAY KIT RF Rheumatoid Factor, IgM test, kit, assay, EIA, enzyme immunoassay |
| Gene, Accession, CAS # | n/a |
| Catalog # | 4046 |
| Price | $435 |
| Order / More Info | RF IgM (Rheumatoid Factor) ELISA from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
EAGLE BIOSCIENCES INC.
EAGLE BIOSCIENCES INC.
20A NW Blvd., Suite 112
Nashua NH 03063
P: 617-419-2019
P: 866-411-8023
F: 617-419-1110
info@EagleBio.com
http://www.EagleBio.com
Profile of EAGLE BIOSCIENCES INC.