Edit |   |
---|---|
Product Name | Bovine Lactoferrin ELISA Kit |
Description | The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the lactoferrin (LF) present in samples reacts with the anti-LF antibodies, which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-LF antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound LF. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of LF in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of LF in the test sample. The quantity of LF in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Sample types: milk/plasma/serum. Range: 3.13 ng/ml - 200 ng/ml. Incubation time: 1 hr 40 mins |
Size | 12 x 8 wells |
Concentration | n/a |
Applications | RUO |
Other Names | kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | LTF61-K01 |
Price | $580 |
Order / More Info | Bovine Lactoferrin ELISA Kit from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |