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Product Name | Tumor Necrosis Factor ligand (Trail) ELISA |
Description | The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Human Tumor Necrosis Factor ligand Superfamily Member 10, TNFSF10 / Trail present in the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Human Tumor Necrosis Factor ligand Superfamily Member 10, TNFSF10 / Trail in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm. Sample types: serum, cell culture supernatant and plasma. Range: 5 ng/l - 1500 ng/l. Sensitivity: 2.49 ng/l. Incubation time: 70 minutes |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | KBH3154 |
Price | $560 |
Order / More Info | Tumor Necrosis Factor ligand (Trail) ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |