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Product Name | Human Apolipoprotein H (ApoH) ELISA |
Description | The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Apo-H present in samples reacts with the anti-Apo-H antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Apo-H antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound Apo-H. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Apo-H in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Apo-H in the test sample. The quantity of Apo-H in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution. Sample types: biological fluids. Range: 3.125 - 200 ng/ml. Incubation time: 90 minutes |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | Apolipoprotein H (ApoH) ELISA test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | APH39-K01 |
Price | $600 |
Order / More Info | Human Apolipoprotein H (ApoH) ELISA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |