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Product Name | functional IGFBP-3 LIA |
Description | The Eagle Biosciences IGFBP-3 LIA Assay Kit utilizes specific and high affinity antibodies for IGFBP-3, the antibodies are immobilized on the microtitre plate. The ligand, biotinylated IGF-I is pre-dispensed in excess into the needed wells. The sample is diluted outside within a special dilution buffer (Sample Buffer PP); all naturally bound IGFs are thereby released from their binding proteins. By adding an aliquot of such diluted sample with free IGFBP-3 to the ligand containing wells, the biotinylated IGF-I occupies all existing specific binding sites of the IGFBP-3. All IGFBP-3 molecules are bound afterwards to the microtitre plate, however by using a Strepavidin-Peroxydase-(POD)-Conjugate only the complexes of IGFBP-3/biotinylated IGF-I are involved in signal generation (in the closing POD-substrate reaction), thus, only functional IGFBP-3 is being quantified. Sample types: serum, plasma. Range: 0.4 - 30 ng/mL. Sensitivity: 0.4 ng/mL. Incubation time: 3.5 hours |
Size | 1 x 96 well |
Concentration | n/a |
Applications | RUO |
Other Names | IGFBP-3, Insulin Growth Factor Binding Protein test, kit, assay, EIA, enzyme immunoassay |
Gene, Accession, CAS # | n/a |
Catalog # | E04A |
Price | $675 |
Order / More Info | functional IGFBP-3 LIA from EAGLE BIOSCIENCES INC. |
Product Specific References | n/a |