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Product Name | EcHECk, ELISA kit |
Description | Range: 1.6 to 100 AU/ml. The EcHECk ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3.5 hours. The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with LPS recognizing IgM EHEC O157:H7. Biotinylated tracer antibody will bind to the captured Human-anti-EHEC O157:H7 IgM. Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody. Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the EcHECk standards (log). |
Size | 1 x 96 det. |
Concentration | n/a |
Applications | RUO |
Other Names | EHEC; Enterohemorrhagic Escherichia coli; serotype O157:H7; Shiga-toxin producing Escherichia coli; STEC |
Gene, Accession, CAS # | n/a |
Catalog # | HK508 |
Price | $2453 |
Order / More Info | EcHECk, ELISA kit from HYCULT BIOTECH |
Product Specific References | n/a |