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Product Name | Factor H, 402H/Y variant detection, Human, ELISA kit |
Description | The HK353 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1.5 hours. The efficient format of 1 plate with 3 (CFH, CFH-H402 and CFH-Y402) x 4 disposable 8-well strips allows free choice of batch size for the assay. Samples and controls are incubated together with peroxidase-conjugated secondond antibody (conjugate) in microtiter wells coated with antibodies recognizing all variants of CFH (colorless), CFH H402 (white) and CFH Y402 (black). During incubation CFH, CFH H402 or CFH Y402 are captured by the solid bound antibody. The conjugated antibodies will bind to the captured CFH variants. The peroxidase conjugated antibody will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. The polymorphism (Y402H) of complement factor H can be determined from the controls. |
Size | 1 x 96 det. |
Concentration | n/a |
Applications | RUO |
Other Names | CFHL1; CFHL3; HF1; HF2; HUS; MGC88246; beta-1-H-globulin |
Gene, Accession, CAS # | n/a |
Catalog # | HK353 |
Price | $1054 |
Order / More Info | Factor H, 402H/Y variant detection, Human, ELISA kit from HYCULT BIOTECH |
Product Specific References | n/a |