MutaPLEX® Enterovirus from IMMUNDIAGNOSTIK AG
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| Product Name | MutaPLEX® Enterovirus |
| Description | MutaPLEX® Enterovirus real time RT-PCR kit contains specific primers, fluorescence-marked probes and additional material for the detection of enterovirus RNA in clinical samples. Human stool or other clinical samples, such as whole blood, plasma, respiratory samples, CSF (cerebrospinal fluid) etc. can be used as starting material for RNA-extraction and following pathogen analysis with MutaLPLEX® Enterovirus. Reverse transcription (RT) of potentially contained viral RNA to cDNA and subsequent amplification of enterovirus-specifc fragments by means of PCR is done in only one step. Target sequence for the detection is within the 5?-untranslated region (5?-UTR) of the genome. Detection of enterovirus amplificates is achieved in real time by hybridization and subsequent hydrolysis of enterovirus-specific fluorescence probes. Their emitted signal is measured (during PCR process) by the optical unit of the real time PCR system in use (ABI: PRISM SDS-, Stratagene: MxPRO-, Corbett Research: RotorGene 3000/ 6000-, Cepheid: SmartCycler- Software, Roche: LC480- software). Enterovirus-specific amplification is measured in FAM-channel (470/ 510 nm). Furthermore, MutaPLEX® Enterovirus real time RT-PCR - kit contains also a separate RNA extraction control. This is added initially prior to the nucleic acid extraction procedure and detected in the follow-up by an independent amplification step. This internal control (IC) allows on the one hand the exposure of errors during RNA extraction procedure and identifies on the other hand a potential inhibition of reverse transcription or PCR. Doing this, the risk for false-negative results is minimized extensively. RNA extraction control (= internal control for PCR) is measured in VIC- (respectively = HEX/ JOE/ TET/ Cy3) channel (530/ 555 nm). |
| Size | 96 tests |
| Concentration | n/a |
| Applications | n/a |
| Other Names | Enterovirus |
| Gene, Accession, CAS # | n/a |
| Catalog # | KG190296 |
| Price | please inquire |
| Order / More Info | MutaPLEX® Enterovirus from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
Profile of IMMUNDIAGNOSTIK AG