IDK® PMN elastase ELISA from IMMUNDIAGNOSTIK AG
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| Product Name | IDK® PMN elastase ELISA |
| Description | In a first incubation step, PMN elastase in the sample is bound to polyclonal rabbit-anti-PMN elastase antibodies, which are immobilised on the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out. In a second incubation step, a monoclonal mouse-anti-PMN elastase antibody is added. This antibody is able to detect both the free and the complexed form with the specific inhibitor (α1-proteinase inhibitor = α1-antitrypsin). The quantification of the bound PMN elastase is carried out by adding an anti-mouse peroxidase-labeled conjugate. Finally, the PMN elastase-antigen-antibody-complex is incubated with the peroxidase substrate, tetramethylbenzidine. An acidic stop solution is then added to terminate the reaction. The colour changes from blue to yellow. The intensity of the yellow colour is directly proportional to the concentration of PMN elastase in the sample. A dose response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the values obtained from the standards. PMN elastase, present in the patient samples, is determined directly from this curve. Range: 0.37 - 10 ng/ml |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | PMN Elastase |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 6840 |
| Price | please inquire |
| Order / More Info | IDK® PMN elastase ELISA from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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