IDK® Quinolinic acid ELISA from IMMUNDIAGNOSTIK AG
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| Product Name | IDK® Quinolinic acid ELISA |
| Description | The assay is based on the method of competitive enzyme linkedimmunoassays. Samples, standards and controls are incubated in wells of amicrotiter plate coated with quinolinicic acid (antigen), together with apolyclonal anti-quinolinicic acid antibody. The free target antigen in thesample competes with the antigen immobilized on the wall of the microtiterwells for the binding of the polyclonal antibodies.In the second incubation step, a peroxidase-conjugated antibody is added toeach microtiter well to detect the anti-quinolinicic acid antibodies. After awashing step to remove the unbound components, the peroxidase substratetetramethylbenzidine (TMB) is added. Finally, the enzymatic reaction isManual IDKR QuinA ELISA19terminated by an acidic stop solution. The color changes from blue to yellowand the absorbance is measured in the photometer at 450 nm. The intensity ofthe yellow color is inverse proportional to the quinolinicic acid concentration inthe sample. This means, high antigen concentration in the sample reduces theconcentration of antibodies bound to the antigen on the plate and lowers thephotometric signal. A dose response curve of absorbance unit (optical density,OD at 450 nm) vs. concentration is generated using the values obtained fromthe standards. Quinolinicic acid present in the patient samples is determineddirectly from this curve. Range: 3-300 µmol/l |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | QuinA |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 7736 |
| Price | please inquire |
| Order / More Info | IDK® Quinolinic acid ELISA from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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