Nitrotyrosine ELISA from IMMUNDIAGNOSTIK AG
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| Product Name | Nitrotyrosine ELISA |
| Description | The assay utilizes the ``sandwich'' technique. Standards, controls and diluted samples which are assayed for nitrotyrosine are added into the wells of a micro plate coated with polyclonal goat anti- nitrotyrosine antibody. During the first incubation step, nitrated proteins are bound by the immobilized primary antibody. Then a peroxidase-conjugated polyclonal goat anti-human serum proteins antibody is added into each microtiter well and a ``sandwich'' of primary antibody - nitrated protein - peroxidase-conjugate is formed. Tetramethylbenzidine is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of nitrotyrosine. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. standard concentration is generated, using the values obtained from the standards. Range: 4.9-400 nM |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | Nitrotyrosine |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 7829 |
| Price | please inquire |
| Order / More Info | Nitrotyrosine ELISA from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
Profile of IMMUNDIAGNOSTIK AG