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Product Name | IDKmonitor® Ustekinumab total ADA ELISA |
Description | This ELISA serves for the determination of antibodies against TNF therapeutic antibody ustekinumab (e.g. STELARA®). During sample preparation, the anti-drug antibodies (ADA) are separated from the therapeutic antibody in order to acquire free ADA. By adding the peroxidase conjugate (peroxidase labelled therapeutic anti body) and the tracer (biotinylated therapeutic antibody), the unmarked therapeutic antibodies are replaced and the marked antibodies can form a complex with the ADA. This complex binds via biotin to the streptavidin coated microtiter plate. It is detected via the peroxidase conjugate with the peroxidase converting the substrate TMB to a blue product. The enzymatic reaction is stopped by adding an acidic solu tion. The samples convert from blue to yellow. The colour change should be meas ured in a photometer at 450nm. The interpretation is made using the cutoff control. |
Size | 97 wells |
Concentration | n/a |
Applications | n/a |
Other Names | Ustekinumab |
Gene, Accession, CAS # | n/a |
Catalog # | K 9667 |
Price | please inquire |
Order / More Info | IDKmonitor® Ustekinumab total ADA ELISA from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |