IDK® PMN elastase ELISA from IMMUNDIAGNOSTIK AG
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| Product Name | IDK® PMN elastase ELISA |
| Description | In a first incubation step, PMN elastase in the sample is bound to polyclonal sheep-anti-PMN elastase antibodies (in excess), which are immobilised on the surface of the microtiter wells (PLATE). To remove all unbound substances, a washing step is carried out. In a second incubation step, a monoclonal mouse-anti-PMN elastase antibody (AB) is added. This antibody is able to detect both the free and the complexed form with the specific inhibitor (α1-proteinase inhibitor = α1-antitrypsin). The quantification of the bound PMN elastase is carried out by adding an anti-mouse peroxidase-labeled conjugate (CONJ). Finally, the PMN elastase - antigen - antibody complex is incubated with the peroxidase substrate, tetramethylbenzidine (SUB). An acidic stop solution (STOP) is then added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of PMN elastase in the sample. Samples are quantified by referring their optical density to a lot-dependent master calibration curve and the use of a calibrator that is run with each test. The combination of two specific antibodies in the PMN elastase ELISA drastically reduces the possibility of false results and offers a reliable diagnostic system to the user. Range: 0.37-10 ng/ml |
| Size | 96 wells |
| Concentration | n/a |
| Applications | n/a |
| Other Names | PMN Elastase |
| Gene, Accession, CAS # | n/a |
| Catalog # | K 6831 |
| Price | please inquire |
| Order / More Info | IDK® PMN elastase ELISA from IMMUNDIAGNOSTIK AG |
| Product Specific References | n/a |
IMMUNDIAGNOSTIK AG
IMMUNDIAGNOSTIK AG
Stubenwald Allee 8a
64625 Bensheim GERMANY
P: 49-6251701900
F: 49-6251849430
info@immundiagnostik.com
http://www.immundiagnostik.com
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