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Product Name | MutaPLEX® GastroSys 1 |
Description | The MutaPLEX® GastroSys1 real time RT-PCR contains specific primers and hydrolysis probes for the detection of the nucleic acids of rotavirus, adenovirus, and norovirus in clinical specimens (e.g. stool samples, vomit), environmental and food samples. The reverse transcription (RT) of viral RNA to cDNA and the subsequent amplification of virus specific fragments are performed in a one-step RT-PCR. The amplification can be detected when specific probes are hydrolysed by the polymerase. The emitted fluorescence is measured in the FAM (norovirus), ROX (rotavirus) and Cy5 (adenovirus) channel. Furthermore, the MutaPLEX® GastroSys1 real time RT-PCR contains a control RNA, which is detected in a second amplification system. Added during RNA extraction, the control RNA allows not only for the detection of RT-PCR inhibition but also detects possible mistakes during RNA extraction. This greatly reduces the risk of false-negative results. The control RNA can also be used solely as internal control by adding it directly to the master mix. The fluorescence of the control RNA is measured in the VIC®/HEX/JOETM/TET channel. |
Size | 96 tests |
Concentration | n/a |
Applications | n/a |
Other Names | GastroSys 1 |
Gene, Accession, CAS # | n/a |
Catalog # | KG198696 |
Price | please inquire |
Order / More Info | MutaPLEX® GastroSys 1 from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |