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Product Name | IDKmonitor® Etanercept drug level ELISA |
Description | This ELISA is designed to determine the quantity of free Etanercept (therapeutic antibody agains TNFα, e. g. ENBREL®) in EDTA plasma or serum samples. In a first incubation step, the free Etanercept from the sample is bound to the specific monoclonal anti-Etanercept antibody coated on the plate. To remove all unbound substances, a washing step is carried out. In a further incubation step, peroxidase-labeled antibody is added. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of free Etanercept in the sample. A dose response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the values obtained from standard. The concentrations of free Etanercept in the samples are determined directly from this curve. Range: 4.15-225 ng/ml |
Size | 96 wells |
Concentration | n/a |
Applications | n/a |
Other Names | Etanercept |
Gene, Accession, CAS # | n/a |
Catalog # | K 9646 |
Price | please inquire |
Order / More Info | IDKmonitor® Etanercept drug level ELISA from IMMUNDIAGNOSTIK AG |
Product Specific References | n/a |